RMCE (Recombination-Mediated Cassette Exchange)
| Detailed Steps | Cost | |
| MiMIC or CRIMIC stock set-up fee | $50 | |
| Inject gene-trap (or protein trap) donor into PhiC31/+; MiMIC or CRIMIC/+ embryos (n = 480) for RMCE | $440 | |
| Set G0 crosses and conduct F1 screen for loss of yellow+ or GFP+ | $100 | |
| Conduct PCR screen to identify F1 animals with the correct orientation of the insertion (20 individual F1 crosses, genomic DNA isolation from F1 founders, and PCR.) | $140 | |
| Establish balanced stocks from positive F1 animals (up to three) | $50 | Total $780 |