Transgenic Services

We offer a wide range of transgenic services, including P-element insertions, site-specific insertion using the PhiC31 system, MiMIC/RMCE events, and CRISPR gene targeting.

P-element Insertions

Although site-specific integration is the preferred method of transgenesis in most cases, P-element transgenics still offer one benefit: variable expression levels of different transgenes due to chromosomal position effects. Therefore, if your experiment requires a wide variety of expression levels, P-element insertions are still worth considering. Otherwise, we strongly encourage clients to use site-specific integration for a number of reasons (see below).

Site-Specific, PhiC31-Mediated Insertions

There are many benefits to using site-specific PhiC31-mediated insertions. First, we can generate transgenic flies carrying insertions well in excess of 100 kb. Therefore, nearly all genes in the genome can be introduced for genetic rescue, structure-function, or gene regulation studies. Second, even if your transgene is relatively small (5) numbers of independent transgenes as needed if comparing P-element insertions. This leads to much more efficient, rapid, and cost-saving experimental designs and greatly simplifies interpretation of transgenic data. Finally, when using site-specific insertion, you know exactly where your transgene is in the genome, greatly simplifying subsequent genetics, such as recombining the transgene with other mutations or transgenes on the same chromosome. We are currently offering the docking sites at no additional charge:

ZH-2A: (X) 2A3
attP18: (X) 6C12
su(Hw)attP8: (X) 8E10
VK37: (2L) 22A3
JK22C: (2L) 22C3
attP40: (2L) 25C6
JB38F: (2L) 38F1
su(Hw)attP5: (2R) 50F1
ZH-51C: (2R) 51C1
attP1: (2R) 55C4
VK22: (2R) 57F5
VK01: (2R) 59D3
VK31: (3L) 62E1
VK33: (3L) 65B2
JK65C: (3L) 65C3
JK66B: (3L) 66B4
attP2: (3L) 68A4
JK73A: (3L) 73A9
VK05: (3L) 75A10
ZH-86Fb: (3R) 86F8
VK40: (3R) 87B10
su(Hw)attP1: (3R) 87B13
VK27: (3R) 89E11
VK20: (3R) 99F8